Journal: BMC Pharmacology & Toxicology

Article Title: Can vildagliptin protect against radiation-induced premature ovarian failure? Insights into the AMPK and AKT signaling pathways

doi: 10.1186/s40360-025-00903-5

Figure Lengend Snippet: Immunohistochemical detection of Nrf2 expression (x 400). ( A , B ) Control and vilda alone groups showed minimal immunostaining, respectively. ( C ) Ovaries from irradiated rats showed extensive brown immunostaining (increased cytoplasmic Nrf2). ( D ) Vilda co-administration resulted in a marked reduction in cytoplasmic Nrf2. ( E ) Quantitative image analysis for Nrf2 immunostaining was expressed as optical density (O.D.). ( F ) Scoring of the immunostaining considering both the staining intensity and the percentage of positive cells

Article Snippet: The slides were then blocked with 5% bovine serum albumin in tris-buffered saline for 2 h. The sections were then immunostained with Nrf2 primary antibody: rabbit polyclonal anti-rat Nrf2 antibody (ABclonal, Cat No. A0674) at a concentration of 1 μg/ml containing 5% bovine serum albumin in tris-buffered saline and incubated overnight at 4 °C.

Techniques: Immunohistochemical staining, Expressing, Control, Immunostaining, Irradiation, Staining

Journal: Experimental and Therapeutic Medicine

Article Title: Therapeutic effect of irbesartan combined with atorvastatin calcium in the treatment of rats with coronary heart disease

doi: 10.3892/etm.2018.6669

Figure Lengend Snippet: Primer sequences used in PCR reaction.

Article Snippet: Skim milk powder (BD Biosciences, San Jose, CA, USA), rabbit anti-rat sPLA2-V polyclonal antibody (cat no. 16009-1-AP; 1:1,000) and goat anti-rabbit-HRP secondary polyclonal antibody (cat. no. SA00001-2; 1:800) were purchased from Wuhan Sanying Biotechnology, Wuhan, China.

Techniques: Sequencing

Journal: Experimental and Therapeutic Medicine

Article Title: Therapeutic effect of irbesartan combined with atorvastatin calcium in the treatment of rats with coronary heart disease

doi: 10.3892/etm.2018.6669

Figure Lengend Snippet: Comparisons of expression level of PLA2-V mRNA in myocardium and aortic trunk among groups. RT-PCR results showed that expression levels of sPLA2-V mRNA in apex and aorta of model groups were significant higher than those of irbesartan group , atorvastatin calcium group and combination group (*p<0.05). In addition, expression levels of sPLA2-V mRNA in apex and aorta of combination group were significantly lower than those in irbesartan and atorvastatin calcium groups (*, # p<0.05). sPLA2-V, type II secretory phospholipase A2-V.

Article Snippet: Skim milk powder (BD Biosciences, San Jose, CA, USA), rabbit anti-rat sPLA2-V polyclonal antibody (cat no. 16009-1-AP; 1:1,000) and goat anti-rabbit-HRP secondary polyclonal antibody (cat. no. SA00001-2; 1:800) were purchased from Wuhan Sanying Biotechnology, Wuhan, China.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: Therapeutic effect of irbesartan combined with atorvastatin calcium in the treatment of rats with coronary heart disease

doi: 10.3892/etm.2018.6669

Figure Lengend Snippet: Comparisons of expression level of PLA2-V protein in myocardium and aortic trunk among groups. Western blot analysis results showed that expression levels of sPLA2-V protein in apex and aorta of model group were significant higher than those of irbesartan group , atorvastatin calcium group and combination group (*p<0.05). In addition, expression levels of sPLA2-V protein in apex and aorta of combination group were significantly lower than those in irbesartan and atorvastatin calcium groups (*, # p<0.05). sPLA2-V, type II secretory phospholipase A2-V.

Article Snippet: Skim milk powder (BD Biosciences, San Jose, CA, USA), rabbit anti-rat sPLA2-V polyclonal antibody (cat no. 16009-1-AP; 1:1,000) and goat anti-rabbit-HRP secondary polyclonal antibody (cat. no. SA00001-2; 1:800) were purchased from Wuhan Sanying Biotechnology, Wuhan, China.

Techniques: Expressing, Western Blot

Journal: Frontiers in Molecular Neuroscience

Article Title: Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models

doi: 10.3389/fnmol.2017.00128

Figure Lengend Snippet: Immunoblotting analyses of PrP Sc and repressor element 1-silencing transcription (REST) in brain tissues of normal control and scrapie 263K-infected hamsters. (A) Western blot analysis of PK-resistant PrP. (B,C) Immunoblotting density of REST was normalized to β-actin and values are expressed as fold changes relative to the 263K-infected hamsters. Data are presented as mean ± SD, n = 10. ∗∗ P < 0.01 vs. the normal control. Statistical significance was evaluated using Student’s t -test.

Article Snippet: Rabbit polyclonal anti-rat β-actin antibody (Cat No.: AP0060) (1:1000), and goat anti-rabbit IgG (H&L)-HRP secondary antibody (Cat No.:BS13278) (1:5000) were purchased from Bioworld Technology (Nanjing, China Bioworld Technology, Inc.).

Techniques: Western Blot, Infection